Improved real-time PCR assays for the detection of fecal indicator bacteria in surface waters with different instrument and reagent systems
نویسندگان
چکیده
منابع مشابه
Improved real-time PCR assays for the detection of fecal indicator bacteria in surface waters with different instrument and reagent systems.
Previously reported and redesigned primer and probe assays were evaluated for the quantitative analysis of the fecal indicator bacterial groups, Enterococcus and Bacteroidetes with three real-time PCR instrument and reagent systems. The efficiency and sensitivity of the original assays varied between systems in analyses of DNA extracts from pure cultures of Enterococcus faecalis and Bacteroides...
متن کاملFungi Occurrence Assessment in Drinking Water Distribution Systems and Its Relationship with Fecal Indicator Bacteria
Introduction: As recommended by World Health Organization, consumption of the fungal contaminated water does not cause to serious infection, but may lead to healthy or aesthetic problems. The aim of this research was to assess the occurrence of fungi in water and its relationship with fecal indicator bacteria. Materials and Methods: 110 water samples were collected from different location of w...
متن کاملthe effect of task cognitive complexity and time limit on the performance of the learners with different proficiency levels in second language writing skill
this study investigated the effects of manipulating the cognitive complexity of tasks along +/- few elements and the time limits on l2 learners writing performance. to conduct the study, 60 iranian efl learners with two levels of proficiency, low and high intermediate, were selected and assigned to three groups based on the time devoted to task completion. the participants performed both a simp...
15 صفحه اولInterlaboratory comparison of real-time PCR protocols for quantification of general fecal indicator bacteria.
The application of quantitative real-time PCR (qPCR) technologies for the rapid identification of fecal bacteria in environmental waters is being considered for use as a national water quality metric in the United States. The transition from research tool to a standardized protocol requires information on the reproducibility and sources of variation associated with qPCR methodology across labor...
متن کاملMulticenter comparison of different real-time PCR assays for quantitative detection of Epstein-Barr virus.
Quantification of Epstein-Barr virus (EBV) in peripheral blood is important for the diagnosis and management of serious EBV diseases, including posttransplant lymphoproliferative disorder. A variety of PCR-based methods are currently in use; however, there is little information on their comparability. This study assessed the relative performance of different quantitative assays. A multicenter c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Water and Health
سال: 2008
ISSN: 1477-8920,1996-7829
DOI: 10.2166/wh.2008.022